altoslabs
Research Specialist / Scientist, Metabolic Phenotyping (In Vitro & In Vivo)
At a Glance
- Location
- San Francisco Bay Area, California, United States
- Employment
- employment_required
- Experience
- Up to 2 years
- Compensation
- ty, CA: Research Specialist : $129,500 - $166,000 Scientist I, Biological Scien
- Posted
- 2026-07-27T13:10:58-04:00
Key Requirements
Required Skills
Domain Knowledge
- Automation
- Education
- Engineering
- Regulatory
Requirements
workflows, including direct experience designing and executing
rodent studies (e.g., animal handling, dosing, metabolic phenotyping, and tissue collection), with a strong background in Institutional Animal Care and Use Committee (IACUC) protocol compliance.
Experience preparing biological samples for downstream metabolomics, lipidomics, or genomics pipelines.
Demonstrated experience with immunofluorescence/immunohistochemistry staining and fluorescence, confocal, or high-content microscopy of cells or metabolic tissue sections.
rodent experience specifically in preclinical metabolic disease models (e.g., obesity, diabetes, metabolic dysfunction-associated steatohepatitis [MASH], or mitochondrial dysfunction).
Hands-on experience setting up, running, and analyzing data from Agilent Seahorse XF instruments (or equivalent extracellular-flux/respirometry platforms).
Responsibilities
rodent metabolic studies in collaboration with the team, including animal handling, dosing (intraperitoneal, intravenous, oral), metabolic phenotyping (glucose- and insulin-tolerance tests, CLAMS for metabolic monitoring), and endpoint tissue collection optimized for multi-omics, bioenergetic, and histological analysis.
immunofluorescence and immunohistochemistry on cultured cells and metabolic tissues (e.g., liver, adipose, muscle), and operate fluorescence, confocal, or automated high-content imaging systems to visualize lipid droplets, mitochondrial morphology, and protein localization.
metabolic flux assays on the Agilent Seahorse XF analyzer, and prepare cellular and tissue extracts for downstream mass spectrometry-based metabolomics and lipidomics and for genomics workflows (RNA/DNA isolation, quantitative PCR).
Analyze and interpret
bioenergetic, imaging, and phenotyping data (Wave, ImageJ/FIJI, GraphPad Prism), drawing accurate conclusions that guide the next round of experiments.
primary metabolic cell cultures and develop, optimize, and scale the throughput of cell-based assays.